The structured abstract should be written in English with no more than 250 words. It should include background and objectives, methods, results, conclusions, and a maximum of six (6) keywords. Chemical nomenclature should conform to IUPAC rules and measurements should be in SI units.
NB: Section headers may be omitted and the sections can be combined into a single paragraph. The sections were highlighted here as an example.
Immunotherapy using slow-cycling tumor cells prolonged overall survival of tumor-bearing mice
Background: Despite considerable progress in the development of anticancer therapies, there is still a high mortality rate caused by cancer relapse and metastasis. Dormant or slow-cycling residual tumor cells are thought to be a source of tumor relapse and metastasis, and are therefore an obstacle to therapy. In this study, we assessed the drug resistance of tumor cells in mice, and investigated whether vaccination could promote survival.
Methods: The mouse colon carcinoma cell line CT-26 was treated with 5-fluorouracil to assess its sensitivity to drug treatment. Mice with colon tumors were immunized with inactivated slow-cycling CT-26 cells to estimate the efficacy of this vaccine.
Results: We identified a small population of slow-cycling tumor cells in the mouse colon carcinoma CT-26 cell line, which was resistant to conventional chemotherapy. To inhibit tumor recurrence and metastasis more effectively, treatments that selectively target the slow-cycling tumor cells should be developed to complement conventional therapies. We found that drug-treated, slow-cycling tumor cells induced a more intense immune response in vitro. Moreover, vaccination with inactivated slow-cycling tumor cells caused a reduction in tumor volume and prolonged the overall survival of tumor-bearing mice.
Conclusions: These findings suggest that targeting of slow-cycling tumor cells application using immunotherapy is a possible treatment to complement traditional antitumor therapy.
Keywords: cancer relapse, drug resistance, slow-cycling tumor cells, tumor vaccine
Sun Q, Zhong Y, Wu F, Zhou C, Wang D, Ma W, et al. Immunotherapy using slow-cycling tumor cells prolonged overall survival of tumor-bearing mice. BMC Medicine. 2012 Dec; 10(172).